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Identification
CAS Number
24-1-1060
Name
Bovin beta-1,4-Galactosyltransferase 1 (Y289L) /Bovin B4GALT1 (Y289L) CAS 24-1-1060
Synonyms
Bovine B4GALT1 (Y289L)
Molecular Weight
50.8 kDa
Properties
Appearance
White freeze-dried powder
Safety Data
Symbol
Signal Word
WGK Germany
3
MSDS Download
Specifications and Other Information of Our Bovin beta-1,4-Galactosyltransferase 1 (Y289L) /Bovin B4GALT1 (Y289L) CAS 24-1-1060
Identification Methods
Source
HEK293 cell
Storage Buffer
20mM Tris-HCl(PH7.4), 200 mM NaCl, 20% Glycerin
Enzyme Activity
>1000 U/mg
Unit Definition
Amount of enzyme required to transfer 1 nmol GalNAz to GlcNAc per min at 37°C (mg)
Shelf Life
1 year
Storage
Store at -20℃ and away from light
Known Application
Bovine B4GALT1(Y289L) is a mutant galactosyltransferase capable of efficiently transferring GalNAc or GalNAz onto terminal GlcNAc residues. This enzyme is particularly useful in the synthesis of antibody-drug conjugates (ADCs), which are applied in targeted cancer therapies and other biomedical applications.
General View of Documents
Reactive of Bovin beta-1,4-Galactosyltransferase 1 (Y289L) CAS 24-1-1060
1.Reaction System:50 mM Tris-HCl (pH 7.5), 6 mM MnCl₂
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2.Reaction Reagents:① UDP-GalNAz: Antibody (molar ratio) = 300:1② Enzyme addition: Enzyme/Antibody = 5%
3.Prepare the reaction mixture according to the conditions above and incubate at 37°C for 48 hours. Subsequently, place the reaction mixture at -15 to -25°C to terminate the reaction and proceed with sample analysis.
Links
This product is developed by our R&D company Watson Bio Limited (https://www.watson-bio.com/).
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