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5(6)-Nitrofluorescein CAS 78512-32-0
- Identification

- Properties

- Safety Data

- Specifications & Other Information

- Links

- Quick Inquiry

Identification

CAS Number

78512-32-0

Name

5(6)-Nitrofluorescein

Synonyms

3′,6′-Dihydroxy-5-nitro-3H-spiro-3-on3′,6′-Dihydroxy-5-nitro-3H-spiro-3-one3′,6′-Dihydroxy-5-nitro-3H-spiro-3-one3′,6′-dihydroxy-5-nitro-3H-spiro-3-oneSpiro(isobenzofuran-1(3H),9′-(9H)xanthen)-3-one, 3′,6′-dihydroxy-5-nitro-Spiroxanthen]-3-one, 3′,6′-dihydroxy-5-nitro-3′,6′-dihydroxy-5-nitrospiro-3-one3′,6′-DIHYDROXY-5-NITROSPIRO-3-ONE3′,6′-dihydroxy-6-nitrospiro-1-one4-NITROFLUORESCEIN5-Nitrofluorescein5-Nitrofluorescein (isomer I)MFCD00059693MFCD00059694MFCD00135108Nitrofluorescein, isomer 1Spiroxanthen]-3-one,3′,6′-dihydroxy-5-nitro-

SMILES

O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N+)cc21

StdInChI

InChI=1S/C20H11NO7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)20(15)14-4-1-10(21(25)26)7-13(14)19(24)28-20/h1-9,22-23H

StdInChIKey

UACQOMKZFGNRBL-UHFFFAOYSA-N

Molecular Formula

C20H11NO7

Molecular Weight

377.308

MDL Number

MFCD00059693

Properties

Appearance

Yellow solid

Safety Data

RIDADR 

NONH for all modes of transport

Specifications and Other Information of Our 5(6)-Nitrofluorescein CAS 78512-32-0

Identification Methods

HNMR, HPLC

Purity

97% min

Shelf Life

1 year

Storage

Under room temperature away from light

Known Application

- Fluorescent probe developmentDue to its adjustable fluorescence response at specific wavelengths, 5(6)-Nitrofluorescein is often used to develop fluorescent probes and molecular markers, especially in bioimaging or analytical chemistry. Nitro substitution will quench fluorescence and can be used to construct "on/off" probes (such as indicators for enzyme reaction monitoring or redox reactions).

- Biolabeling / covalent coupling reactionIt can react with amino compounds through its carboxyl group to form a stable covalent bond, which is used for labeling and tracking biological macromolecules such as proteins and nucleic acids. It is used in conjunction with fluorescence microscopy, flow cytometry, etc.

- Spectroscopic researchIt is used as a model compound to study spectral properties such as intermolecular energy transfer, electronic effects, and changes in fluorescence quantum yield.

- Used to synthesize more complex fluorescent dyes or sensorsIt is an intermediate or starting material for many fluorescent derivatives (such as FRET probes, reactive oxygen detection dyes, etc.).

General View of Documents

HNMR of 5(6)-Nitrofluorescein CAS 78512-32-0

Links

This product is developed by our R&D company Watson Bio Ltd (https://www.watson-bio.com/).

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly. https://www.watson-int.com/56-nitrofluorescein-cas-78512-32-0/

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