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LLCD CAS 9001-60-9
- Identification

- Properties

- Safety Data

- Specifications & Other Information

- Links

- Quick Inquiry

Identification

CAS Number

9001-60-9

EC

1.1.1.27

Name

LLCD, L-Lactic dehydrogenase

Source

Microorganism

Molecular weight

38 kDa (SDS-PAGE)

EINECS

232-617-8

Properties

Appearance

White amorphous powder

Isoelectric point

6.2

Michaelis constant

1.3×10 M(Pyruvate)-6 4.0×10 M(NADH)

Safety Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Specifications and Other Information of Our LLCD CAS 9001-60-9

Specific activity

≥ 300U/mg protein

Unit definition

One unit will convert one micromole of pyruvate to L-lactate per min at pH 7.4 at 25℃.

Optimum pH

6.5

Optimum temperature

45℃

pH Stability

4.5~10.0 (37℃,1hr)

Thermal stability

< 50℃ (pH 7.4, 15min)

Inhibitors

Co ,Cu ,Fe ,Ni ,Zn ,NEM,SDS,Proclin

Effect of Various Chemicals on L-LDH

The enzyme dissolved in 50mM Tris-HCl buffer, pH 7.5 (20U/ml) was incubated with each chemical at 37℃ for 2hr

ChemicalConcn.（mM）Residual activityNone2.0100%CaCl22.0101%CoCl22.050%CuSO42.00%FeCl32.027%MgSO42.094%MnSO42.096%NiCl22.078%ZnSO42.00%K4Fe(CN)62.098%BME2.098%NME2.079%EDTA5.0102%NaN320.0101%Proclin0.045%58%Na-cholate0.10%109%SDS0.05%0%Triton X-1000.10%113%Tween 200.10%115%

Shelf Life

2 years

Storage

Store at -20℃ for long time.

Known Application

For biochemical research, determination of pyruvate, diagnosis of leukemia and myocardial infarction.

Links

This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly.
https://www.watson-int.com/llcd-cas-9001-60-9/

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