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Identification
CAS Number
9001-66-5
Name
Monoamine Oxidase
EC Number
1.5.3 (Recombinant from microorganism)
Molecular Formula
C7H15N2O6P
Molecular Weight
55 kDa (SDS-PAGE)
EINECS
232-623-0
Safety Data
WGK Germany
3
Specifications and Other Information of Our Monoamine Oxidase CAS 9001-66-5
Specification StandardsAppearanceYellow amorphous powder, lyophilizedProtein purity≥90% (from SDS-PAGE)Activity≥4 U/mg solidCatalase≤0.001%ATPase≤0.005%Glucose oxidase≤0.03%Cholesterol oxidase≤0.003%
General Assay Characteristics
MethodEnzymatic/RATE A/EReagentsReady to useCalibratorsLevel 1-0.0 U/L; Level 1-50 U/LCalibration Stability28 daysSample TypeEDTA,SST,Serum,Heparinised plasmaAssay Range0-200U/L undilutedStandardisation
Assay Principle
The assay is based on the increase in absorbance at 555 nm as the formation of quinoneimine dye proceeds in the forward reactions.
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of H2O2 per min under the conditions described below.
Proposed MAO Recipe
Reagent I
CompositionConcentrationCatalog NumberTris-HCl buffer50mMNADH0.45mM CS001α-Ketoglutarate15mMDetergent, Preservative
Reagent II
CompositionConcentrationCatalog NumberTris-HCl buffer50mM Benzylamine 10mM Detergent,Preservative
Major reagent ingredients made by Assay Specilist Lab are list on right
Standard&Control
Home Made: MAO in buffer
Reaction curve chart
Known Application
The enzyme is useful for the determination of fructosyl-L-amino acid.
Links
This product is developed by our R&D company Ulcho Biochemical Ltd(http://www.ulcho.com/), and here is the corresponding linkhttp://www.ulcho.com/monoamine-oxidase-cas-9001-66-5/
Quick Inquiry
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https://www.watson-int.com/monoamine-oxidase-cas-9001-66-5/
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