- Identification
- Properties
- Safety Data
- Specifications & Other Information
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- Quick Inquiry
Identification
CAS Number
UENA-0247
Name
Diaphorase
EC
1.6.5.2
Organism species
Microorganism
Source
Recombinant expressed by E.coli
Formulation
≥160U/mg yellowish amorphous lyophilized power. Refer to the COA and the bottle logo, or according to customer's special requirement.
Molecular mass
~26kDa by SDS-PAGE
Properties
Appearance
Pale yellow amorphous lyophilized powder
Safety Data
RIDADR
NONH for all modes of transport
Specifications and Other Information of Our Diaphorase CAS UENA-0247
Protein purity
＞90% by SDS-PAGE (Fig 1).
Unit definition
One unit is defined as the amount of enzyme which oxidizes one micromole NADH per minute under the conditions of pH7.0 at 37℃ as described.The activity concentration (U/L) is calculated by detecting ∆A/min of formazane dye production rate at 550nm optical wavelength. Millimolar extinction coefficient of formazane dye under the assay conditions is 12.4 cm2/micromole.
Enzyme properties
1) Inhibitor: Sodium azide can be used as a preservative for enzyme solution and lyophilized powder without affecting activity and stability, but 0.1% sodium azide in the detection Reagent R1 and Reagent R2 can inhibit the activity about 25%.2) Stabilizer and cofactor: FMN.3) Optimum pH: pH7.0 (Fig 3).4) pH stability: pH5.0~10.0 (Fig 4).5) Optimum temperature: above 60℃ (Fig 5).6) Thermal stability: below 70℃ in PBS, pH7.4 (Fig 6).7) Freeze-Thaw Stability: after 20 freeze-thaw cycles, the activity of liquid was stable (Fig 7).8) Accelerated stability: The activity of enzyme solution and lyophilized power are stable by incubated at 42℃ for 3 days and 37℃ for 14 days (Fig 8 and 9).9) Special activity: ~400U/mg protein.10) Km=0.22mM (NADH).
Storage
Stable at -20℃ for at least 12 months (Fig 2).
Known Application
As an indicator enzyme, DPR is useful for enzymatic determination of D-3-Hydroxybutyrate when coupled with D-3-Hydroxybutyrate Dehydrogenase (HBDH, EC 1.1.1.30) in clinical analysis. DPR is also useful for enzymatic determination of NADH and other dehydrogenases.
Links
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
Quick Inquiry
Fill out our inquiry form and one of our experts will be in touch with you shortly.
https://www.watson-int.com/diaphorase-cas-uena-0247/
- Properties
- Safety Data
- Specifications & Other Information
- Links
- Quick Inquiry
Identification
CAS Number
UENA-0247
Name
Diaphorase
EC
1.6.5.2
Organism species
Microorganism
Source
Recombinant expressed by E.coli
Formulation
≥160U/mg yellowish amorphous lyophilized power. Refer to the COA and the bottle logo, or according to customer's special requirement.
Molecular mass
~26kDa by SDS-PAGE
Properties
Appearance
Pale yellow amorphous lyophilized powder
Safety Data
RIDADR
NONH for all modes of transport
Specifications and Other Information of Our Diaphorase CAS UENA-0247
Protein purity
＞90% by SDS-PAGE (Fig 1).
Unit definition
One unit is defined as the amount of enzyme which oxidizes one micromole NADH per minute under the conditions of pH7.0 at 37℃ as described.The activity concentration (U/L) is calculated by detecting ∆A/min of formazane dye production rate at 550nm optical wavelength. Millimolar extinction coefficient of formazane dye under the assay conditions is 12.4 cm2/micromole.
Enzyme properties
1) Inhibitor: Sodium azide can be used as a preservative for enzyme solution and lyophilized powder without affecting activity and stability, but 0.1% sodium azide in the detection Reagent R1 and Reagent R2 can inhibit the activity about 25%.2) Stabilizer and cofactor: FMN.3) Optimum pH: pH7.0 (Fig 3).4) pH stability: pH5.0~10.0 (Fig 4).5) Optimum temperature: above 60℃ (Fig 5).6) Thermal stability: below 70℃ in PBS, pH7.4 (Fig 6).7) Freeze-Thaw Stability: after 20 freeze-thaw cycles, the activity of liquid was stable (Fig 7).8) Accelerated stability: The activity of enzyme solution and lyophilized power are stable by incubated at 42℃ for 3 days and 37℃ for 14 days (Fig 8 and 9).9) Special activity: ~400U/mg protein.10) Km=0.22mM (NADH).
Storage
Stable at -20℃ for at least 12 months (Fig 2).
Known Application
As an indicator enzyme, DPR is useful for enzymatic determination of D-3-Hydroxybutyrate when coupled with D-3-Hydroxybutyrate Dehydrogenase (HBDH, EC 1.1.1.30) in clinical analysis. DPR is also useful for enzymatic determination of NADH and other dehydrogenases.
Links
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
Quick Inquiry
Fill out our inquiry form and one of our experts will be in touch with you shortly.
https://www.watson-int.com/diaphorase-cas-uena-0247/
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